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Ellenberger and co-staff (8) experienced intuited exactly such a motion of the OB domain after they solved the crystal framework in the much larger human DNA ligase I (HuLig1; 919 amino acids) bound to an adenylylated nick. Their landmark construction was the แทงบอลออนไลน์ง่ายๆ very first to doc the circumferential envelopment in the DNA duplex by a ligase clamp and also the ensuing bending and distortion from the duplex that power The bottom pairs over the three′-OH side on the nick into an RNA-just like a helical conformation (eight). Equivalent distortions ended up observed in the ChVLig·DNA intricate (7).
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